Self-formed adaptor PCR: a simple and efficient method for chromosome walking |
| |
| Authors: | Wang Shiming He Jian Cui Zhongli Li Shunpeng |
| |
| Affiliation: | Department of Microbiology, Nanjing Agricultural University, No 1 Weigang, Nanjing, PR China. |
| |
| Abstract: | We developed a self-formed adaptor PCR (termed SEFA PCR) which can be used for chromosome walking. Most of the amplified flanking sequences were longer than 2.0 kb, and some were as long as 6.0 kb. SEFA PCR is simple and efficient and should have broad applications in the isolation of unknown sequences in complex genomes. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
