Direct somatic embryogenesis and plant regeneration of carnation (Dianthus caryophyllus L.) |
| |
Authors: | A Yantcheva M Vlahova A Atanassov |
| |
Affiliation: | (1) Institute of Genetic Engineering, Plant Biotechnology Research Center, BUL-2232 Kostinbrod 2232, Bulgaria e-mail: dmuije@bgcict.acad.bg Fax: +359-721-4985, BG;(2) De Montfort University, Norman Borlaug Centre for Plant Science Research, Kostinbrod 2232, Bulgaria, BG |
| |
Abstract: | Conditions for efficient direct somatic embryogenesis and plant regeneration of leaf explants from carnation cultivars Lena
(SIM group) and Bulgarian spray cultivars Nasslada, Yanita, Regina and Line 84 were established. Murashige and Skoog (MS)
liquid medium supplemented with 1 mg/l 2,4-dichlorophenoxyacetic acid and 0.2 mg/l 6-benzylaminopurine was used for direct
induction of embryoids without an additional callus phase. The first globular structures were observed after 20 days of cultivation.
Their further development to the torpedo stage was correlated with the presence of polyethylene glycol (PEG 6000). Somatic
embryo maturation was promoted by casein hydrolysate (1000 mg/l) in MS liquid media. The percentage conversion of embryos
and polyembryos to whole plants varied between 10 and 75% among studied cultivars. Plantlets regenerated by this procedure
were morphologically identical to the donor material and developed normally in a greenhouse.
Received: 29 November 1996 / Revision received: 28 April 1997 / Accepted: 28 May 1998 |
| |
Keywords: | Carnation Somatic embryogenesis Regeneration |
本文献已被 SpringerLink 等数据库收录! |
|