Improved 16S rRNA-targeted probe set for analysis of sulfate-reducing bacteria by fluorescence in situ hybridization |
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| Authors: | Lücker Sebastian Steger Doris Kjeldsen Kasper Urup MacGregor Barbara J Wagner Michael Loy Alexander |
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| Institution: | Department of Microbial Ecology, Faculty of Life Sciences, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria. |
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| Abstract: | An updated dataset of in silico specificities for 54 previously published 16S rRNA-targeted oligonucleotides was assembled to provide guidance for reliable fluorescence in situ hybridization (FISH) analysis of sulfate-reducing bacteria. Additionally, six new FISH probes were developed for major deltaproteobacterial taxa, including a probe trio targeting most Deltaproteobacteria and Gemmatimonadetes. |
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