RNAi-based validation of antibodies for reverse phase protein arrays |
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Authors: | Heiko A Mannsperger Stefan Uhlmann Christian Schmidt Stefan Wiemann Özgür Sahin Ulrike Korf |
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Institution: | 1.German Cancer Research Center, Division of Molecular Genome Analysis, Heidelberg,Heidelberg,Germany |
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Abstract: | Background Reverse phase protein arrays (RPPA) have been demonstrated to be a useful experimental platform for quantitative protein profiling
in a high-throughput format. Target protein detection relies on the readout obtained from a single detection antibody. For
this reason, antibody specificity is a key factor for RPPA. RNAi allows the specific knockdown of a target protein in complex
samples and was therefore examined for its utility to assess antibody performance for RPPA applications. |
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