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对硫酸软骨素传统提取方法的改进
引用本文:奚俊,张有森.对硫酸软骨素传统提取方法的改进[J].生物学杂志,2000,17(6):33-34,10.
作者姓名:奚俊  张有森
作者单位:合肥神鹿集团公司技术中心,合肥,230041
摘    要:软骨中硫酸软骨素与蛋白质结合成蛋白多糖,并与胶原蛋白结合在一起。本文采用先高温蒸煮,后加稀碱与酶解相结合提取该药物,TCA沉淀蛋白质后,高岭土吸附,再用氯仿连续反萃取,使产品质量达到优级纯,较其他方法缩短了原工艺流程,提高了纯度,减轻了碱-盐提取所带来的环境污染。

关 键 词:硫酸软骨素  提取方法  传统方法  改进
文章编号:1008-9632(2000)06-0033-02

The improvement on a troditional extraction of the chondroitin sulfate
XI Jun,ZHANG You-sen.The improvement on a troditional extraction of the chondroitin sulfate[J].Journal of Biology,2000,17(6):33-34,10.
Authors:XI Jun  ZHANG You-sen
Abstract:In the cartilage,the chondroitin sulfate is combined with protein as to form protein polysaccharide,and also combined with colagen protein.In this paper we describe such a method of extracting this medicine.which shorten the technological process,raise the purification and lower the environmental pollution which is caused by base-salted extraction compared with other methods.At the beginning,boil and steam the cartilage at the high temperature;At the following,add some base which in low concentration and some enzyme:deposit protein by trichlo acetic acid;adsorpt by Kaolin;reextract continuously by chloroform.At the end of the process,the purity of the products come up to the GR (guarantee reagent).
Keywords:chondroitin sulfate  simple and fast  extraction    
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