| 结核分枝杆菌三种耐药基因的检测方法 |
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| 引用本文： | 杨柳,苏明权,程晓东,郝晓柯,岳乔红,于文彬. 结核分枝杆菌三种耐药基因的检测方法[J]. 生物技术通讯, 2003, 14(6): 563-565 |
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| 作者姓名： | 杨柳 苏明权 程晓东 郝晓柯 岳乔红 于文彬 |
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| 作者单位： | 第四军医大学,西京医院临床分子生物学研究中心,西安,710033 |
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| 摘 要： | 建立3种结核分枝杆菌耐药基因的检测方法，探讨耐药基因突变与耐药性的关系。将58株临床分离株均做聚合酶链反应-单链构象多态性分析(PCR—SSCP)和传统药物敏感试验。结果表明，结核分枝杆菌耐药基因突变与耐药水平有密切联系，绝大多数结核分枝杆菌耐药基因突变发生在高耐药株，少部分在低耐药株发生基因突变。
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| 关 键 词： | 结核分枝杆菌 katG rpoB rpsL 基因突变 PCR-SSCP 耐药基因 检测方法 |
| 文章编号： | 1009-0002(2003)06-0563-03 |
| 修稿时间： | 2003-07-28 |
Detecting methods of the three drug-resistant genes of Mycobacterium tuberculosis |
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| YANG Liu,SU Ming-quan,CHENG Xiao-dong,HAO Xiao-ke,YUE Qiao-hong,YU Wen-bin. Detecting methods of the three drug-resistant genes of Mycobacterium tuberculosis[J]. Letters in Biotechnology, 2003, 14(6): 563-565 |
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| Authors: | YANG Liu SU Ming-quan CHENG Xiao-dong HAO Xiao-ke YUE Qiao-hong YU Wen-bin |
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| Abstract: | To establishment of determining drug-resistant gene, and investigate the relationship between drug-resistance gene mutations and drug-resistance level in Mycobacterium tuberculosis. 58 clinical isolates strains were tested by PCR-SSCP technique and traditional drug susceptibility tests. The results indicated that the gene mutation was in relation with drug-resistance level of M. tuberculosis. The gene mutation rate was higher in high level of resistance isolated strains than in low level of resistance isolated strains. |
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| Keywords: | Mycobacterium tuberculosis drug resistance katG rpoB rpsL PCR-SSCP |
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