Division of Gastroenterology, Department of Medicine, and Department of Physiology, University of Alabama Medical Center, Birmingham, Ala., U.S.A.
Abstract:
The frog gastric mucosa has been shown to be sensitive to amytal. At 2 mM acid secretion was completely inhibited with a rise of resistance, fall in short-circuit current and no significant change in potential difference. Simultaneously there was 75% inhibition of O2 consumption and 50% depression of ATP levels. Dual-beam spectrophotometric studies of intact mucosa with amytal showed a crossover point between NAD+ and FAD. The microsomal NADH oxidase ferricyanide reductase has also been shown to be amytal sensitive. Cl? transport was relatively insensitive to amytal, suggesting a qualitative distinction between the mechanisms underlying the transport of H+ and Cl? in the mucosa. This was further brought out by the effects of anoxia in which H+ transport was inhibited at 5 min but Cl? transport at minimally 20 min following the onset of anoxia.