Identification of two GH18 chitinase family genes and their use as targets for detection of the crayfish-plague oomycete Aphanomyces astaci |
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| Authors: | Gerald Hochwimmer Reinhard Tober Renè Bibars-Reiter Elisabeth Licek Ralf Steinborn |
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| Affiliation: | (1) Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine, Veterinaerplatz 1, 1210 Vienna, Austria;(2) Vetomics Core Facility, University of Veterinary Medicine, Veterinaerplatz 1, 1210 Vienna, Austria;(3) Department of Biochemistry, University of Vienna, Dr.-Bohr-Gasse 9, 1030 Vienna, Austria;(4) Clinic for Avian, Reptile and Fish Medicine, University of Veterinary Medicine, Veterinaerplatz 1, 1210 Vienna, Austria |
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| Abstract: | Background The oomycete Aphanomyces astaci is regarded as the causative agent of crayfish plague and represents an evident hazard for European crayfish species. Native crayfish populations infected with this pathogen suffer up to 100% mortality. The existence of multiple transmission paths necessitates the development of a reliable, robust and efficient test to detect the pathogen. Currently, A. astaci is diagnosed by a PCR-based assay that suffers from cross-reactivity to other species. We developed an alternative closed-tube assay for A. astaci, which achieves robustness through simultaneous amplification of multiple functionally constrained genes. |
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