Simultaneous determination of plasma estrogens, androgens, and progesterone during the human menstrual cycle |
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Authors: | C Dupon A Hosseinian M H Kim |
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Institution: | Department of Obstetrics and Gynecology University of Chicago Hospitals and Clinics Chicago, Illinois 60637 USA |
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Abstract: | A method is described for the purification and quantitation of estradiol-17β (E2), estrone (E1), testosterone (T), androstenedione (A) and progesterone (P) from a single plasma sample extraction. A combination of Sephadex LH-20 column chromatography and thin layer chromatography is used to separate the phenolic and neutral steroids; quantitation of the purified steroids is by radioimmunoassay (E2, E1) and competitive protein binding (T,A,P) techniques. The precision and accuracy of this method is comparable with that reported by others. A preliminary investigation of the temporal relationship between estrogens androgens, progesterone and serum LH during five normal and one short luteal phase menstrual cycle is presented. |
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