Low Temperature Spectrophotometry on Phototransformation of PR in Pelletable Phytochrome of Pea |
| |
Authors: | Manabe Katsushi |
| |
Institution: | Department of Biology, Yokohama City University Seto 22-2, Kanazawa-ku, Yokohama 236, Japan |
| |
Abstract: | Phototransformation of PR to PFR in a 1,0007,000 x gpelletable fraction (17 KP), which was extracted fromdark-grown pea shoots that had been irradiated by red then far-redlight, was studied by low temperature spectrophotometry. Redlight irradiation of PR in 17 KP at 160°Cinduced an absorption increase at 695696 nm with a concomitant,small decrease of PR absorption at 670 nm. These changes werepartially photoreversed when the sample was irradiated subsequentlywith 700-nm light. At 55°C, red light irradiationof PR resulted mainly in bleaching and consequently in a reductionof the PR peak, accompanied by minor absorbance increases around695 nm. The intermediates formed at 165°C by 660-nmlight irradiation partly reverted back to PR or formed a bleachedintermediate (probably the same bleached intermediate describedabove) in the dark, when the pellets were warmed to 60°C.The bleached intermediate was transformed to PFR in the darkat 10°C or above. These characteristics of PR transformation observed in the pelletablephytochrome were essentially the same as those observed in invivo or soluble phytochrome. (Received December 24, 1982; Accepted July 28, 1983) |
| |
Keywords: | |
本文献已被 Oxford 等数据库收录! |
|