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Differential Exoproteome Analysis of Two Corynebacterium pseudotuberculosis Biovar Ovis Strains Isolated from Goat (1002) and Sheep (C231) |
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| Authors: | Wanderson M. Silva Núbia Seyffert Alessandra Ciprandi Agenor V. Santos Thiago L. P. Castro Luis G. C. Pacheco Debmalya Barh Yves Le Loir Adriano M. C. Pimenta Anderson Miyoshi Artur Silva Vasco Azevedo |
| Affiliation: | 1. Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antonio Carlos, 6627, Pampulha, CP 486, Belo Horizonte, MG, CEP 31.270-901, Brazil 2. Instituto de Ciências Biológicas, Universidade Federal do Pará, Rua Augusto Corrêa, 01, Guamá, Belém, PA, Brazil 3. Instituto de Ciências da Saúde, Universidade Federal da Bahia, Av. Reitor Miguel Calmon, s/n, Vale do Canela, Salvador, BA, CEP 40110100, Brazil 7. Centre for Genomics and Applied Gene Technology, Institute of Integrative Omics and Applied Biotechnology (IIOAB), Purba Medinipur, WB, 721172, India 5. INRA, UMR1253 STLO, 35042, Rennes, France 6. Agrocampus Ouest, UMR1253 STLO, 35042, Rennes, France 4. Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antonio Carlos, 6627, Pampulha, CP 486, Belo Horizonte, MG, CEP 31.270-901, Brazil |
| Abstract: | Corynebacterium pseudotuberculosis is the etiologic agent of caseous lymphadenitis a chronic infectious disease affecting small ruminants. The 2D-DIGE technique was used to compare the exoproteomes of two C. pseudotuberculosis biovar ovis strains isolated from goat (strain 1002) and sheep (strain C231). Seventeen proteins differentially produced were identified here. Nine proteins appeared over-produced in the exoproteome of 1002 goat strain and 8 in that of C231 sheep strain. These proteins were related to various biological functions, such as the cell envelope, respiratory metabolism and proteolysis. This proteomic analysis revealed strain-specific exoproteins although each of the corresponding genes was found in both strain genomes. Such differential expression pattern may reflect inter-strain differences in adaptation to a specific host, in pathogenicity and or in antigenicity of this pathogenic bacterium. |
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