Molecular phylogeny of four selected species of the strictly anamorphic genus <Emphasis Type="Italic">Thysanophora</Emphasis> using nuclear ribosomal DNA sequences |
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Authors: | S Iwamoto S Tokumasu Y Suyama M Kakishima |
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Institution: | (1) Doctoral Degree Program in Agricultural Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan Tel. +81-268-74-2002; Fax +81-268-74-2016 e-mail: susumu.iwamoto@nifty.ne.jp, JP;(2) Sugadaira Montane Research Center, University of Tsukuba, Nagano, Japan, JP;(3) Graduate School of Agricultural Science, Tohoku University, Miyagi, Japan, JP;(4) Institute of Agriculture and Forestry, University of Tsukuba, Ibaraki, Japan, JP |
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Abstract: | To estimate the phylogenetic position of the strictly anamorphic genus Thysanophora among the class Ascomycetes sensu Kirk et al. and to examine the phylogenetic relationships among T. penicillioides and other Thysanophora species, 18S and 28S rDNA (D1 and D2 regions) sequences of 22 strains of four known and two unidentified Thysanophora species were determined and phylogenetically analyzed. The 18S rDNA analysis suggested that all Thysanophora species examined were members of Eurotiomycetidae, Eurotiales, Trichocomaceae. The 28S rDNA analysis indicated that these
species were clustered together with Chromocleista, Eupenicillium, Geosmithia, and Penicillium assignable to three subgenera – Aspergilloides, Furcatum, and Penicillium. In the Eupenicillium lineage, a monophyly of T. penicillioides, T. longispora, T. taxi, T. canadensis, and T. cf. canadensis was supported by comparatively high bootstrap values. However, the ex-type strain and two strains of T. longispora isolated in Japan were of different phylogenetic positions. Thysanophora sp. was positioned at the base of the Thysanophora clade, although it was not supported by significant bootstrap values. From the results of this study, we consider that two
anamorphic genera, Penicillium and Thysanophora, are clearly distinct in morphology but that they are not phylogenetically separable.
Received: August 13, 2001 / Accepted: January 11, 2002 |
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Keywords: | Eurotiales Molecular phylogeny Penicillium rDNA Thysanophora |
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