Fed-batch culture for L-lysine production via on-line state estimation and control |
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| Authors: | Y. -C. Liu W. -T. Wu J. -H. Tsao |
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| Affiliation: | (1) Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan R.O.C.;(2) Union Chemical Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan R.O.C.;(3) Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan R.O.C.;(4) Union Chemical Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan R.O.C. |
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| Abstract: | Computer application for fed-batch culture of Brevibacterium flavum for L-lysine production has been developed. The organisms are auxotrophic mutants for L-homoserine and are resistant to S-(2-aminoethyl)-L-cysteine. Adaptive control is applied for substrate addition. The sugar concentration is estimated using online respiratory measurement. During the period of fed-batch culture, the total sugar concentration is maintained at a given value. The cultivation strategy results in high productivity and high conversion yield. |
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