Distribution of a novel cardioactive neuropeptide (CCAP) in the nervous system of the shore crab Carcinus maenas |
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| Authors: | J Stangier C Hilbich H Dircksen R Keller |
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| Affiliation: | 1. Institut für Zoophysiologie, Rheinische Friedrich Wilhelms-Universität, D-5300 Bonn, Federal Republic of Germany;1. Institut für Molekularbiologie (ZMBH), Universität Heidelberg D-6900 Heidelberg, Federal Republic of Germany;1. Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya, Kuala Lumpur, Malaysia;2. Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia;3. Faculty of Arts, Computing and Creative Industry, Sultan Idris Education University, Tanjung Malim, Perak, Malaysia;4. Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;1. Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Rua Prof. Orlando Marques de Paiva, 87 – Cidade Universitária, 05508-270, São Paulo, Brazil;1. Department of Ecology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, P.O. Box: 76315-117, Kerman 7631818356, Iran;2. Department of Geology, Faculty of Sciences, Shahid Bahonar University of Kerman, P.O. Box: 76169133, Kerman 7619614111, Iran;1. Department of Integrative Zoology, University of Vienna, Althanstraße 14, A-1090 Vienna, Austria;2. Department of Biology, Zoology/Developmental Biology, University of Osnabrück, Barbarastraße 11, D-49069 Osnabrück, Germany;1. Dept. of Biological Sciences, The George Washington University, 2023 G St. NW, Washington, DC 20052, USA;2. Dept. of Scientific Computing, Florida State University, Tallahassee, FL 32306-4120, USA;3. Dept. of Biological Science, Florida State University, Tallahassee, FL 32306-4295, USA;4. Dept. of Biology, The Graduate School and University Center, The City University of New York, 365 5th Ave., New York, NY 10016, USA;5. Dept. of Biology, The College of Staten Island, The City University of New York, 2800 Victory Blvd., Staten Island, NY 10314, USA |
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| Abstract: | A radioimmunoassay (RIA) for the recently discovered crustacean cardioactive peptide (CCAP) has been developed and used to determine contents of CCAP in different parts of the nervous system of the shore crab Carcinus maenas. Immunoreactive material was detected throughout the nervous system. In contrast to the main ganglia which contained low levels of approximately 1.4 pmol CCAP/mg protein (brain and thoracic ganglion), a high concentration was found in a neurohemal structure, the pericardial organs (PO) (868 pmol/mg protein). A predominantly neurohormonal role of CCAP thus suggested is further supported by in vitro release studies. Incubation of POs in high (K+) saline showed that CCAP is secretable in considerable amounts by a Ca++-dependent release mechanism. |
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