蛋白质组学研究中的分离分析技术及其研究进展

在后基因组时代,蛋白质组学成为新的研究热点。蛋白质组学的研究目标是为复杂蛋白质样品建立一个高通量、大规模、自动化的分离分析技术平台,从而实现准确、快速地筛选功能蛋白质。蛋白质的分离分析在蛋白组学研究中起着非常重要的作用。本文主要综述在蛋白质组学研究中二维凝胶电泳、毛细管电泳及其与质谱联用、多维液相分离技术及其与质谱联用和蛋白质芯片等高效分离分析技术的应用研究进展。

Advances of Separation and Analytical Techniques for Proteomics Research

In post-genomic era,proteomics becomes the focus of research.The establishment of a large-scale,high-throughput,automation of separation and analytical techniques platform is the aim of technology development of proteomics research,which could achieve a rapid,accurate screening of functional protein from complex protein samples.The review focuses on the new progress of high-performance separation and analysis techniques which are applied to the field of proteomics increasingly,such as two-dimensional gel electrophoresis,capillary electrophoresis coupled with mass spectrometry,multidimensional liquid chromatography interfaced on line with mass spectrometry and proteinchip.