| 6种细菌菌数测定培养基的优化 |
| |
| 引用本文： | 金自伟,王志,代俊,李啸,汪扬,陈雄. 6种细菌菌数测定培养基的优化[J]. 氨基酸和生物资源, 2014, 36(1): 55-59 |
| |
| 作者姓名： | 金自伟 王志 代俊 李啸 汪扬 陈雄 |
| |
| 作者单位： | 1. 湖北工业大学生物工程学院,湖北武汉,430068;2. 安琪酵母股份有限公司酵母功能湖北省重点实验室,湖北宜昌,433003;3. 湖北省食品药品监督检验研究院,湖北武汉,430064 |
| |
| 摘 要： | 考察平板计数琼脂(PCA)中不同来源的酵母浸粉对6种细菌总数测定的影响,并对培养基中酵母浸粉和胰蛋白胨的配比及含量进行了优化。优化后的培养基组分为胰蛋白胨3.0 g·L-1,酵母浸粉3.0 g·L-1,葡萄糖1.0 g·L-1,琼脂15.0 g·L-1。与市售平板计数琼脂相比,能检出的细菌总数提高6.9%。
|
| 关 键 词： | 细菌总数 培养基优化 酵母浸粉 胰蛋白胨 |
Optimization of Medium for the Determination of Total Count for Six Bacteria |
| |
| Affiliation: | ,College of Bioengineering,Hubei University of Technology,Hubei Province Key Laboratory of Yeast Function,Angel Yeast Co.,Ltd,Hubei Institute for Food and Drug Control |
| |
| Abstract: | The effects of different yeast extracts on the determination of total bacterial count were investigated for 6 bacteria. The proportion and content of yeast extract and tryptone were also optimized. The optimal medium contained tryptone 3.0 g·L-1,yeast extract 3.0 g·L-1,glucose 1.0 g·L-1,agar 15.0 g·L-1. The total bacterial count of optimal medium would be 6.9% higher than PCA. |
| |
| Keywords: | total bacterial count medium optimization yeast extract tryptone |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
