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来源于解淀粉芽胞杆菌的芸薹根肿菌抑菌活性物质的分离与鉴定
引用本文:刘晓艳,张薇,闵勇,张亚妮,张志刚,江爱兵,杨自文. 来源于解淀粉芽胞杆菌的芸薹根肿菌抑菌活性物质的分离与鉴定[J]. 氨基酸和生物资源, 2014, 36(1): 39-43
作者姓名:刘晓艳  张薇  闵勇  张亚妮  张志刚  江爱兵  杨自文
作者单位:湖北省农业科学院湖北省生物农药工程研究中心,湖北武汉,430064;湖北生物科技职业学院园艺园林学院,湖北武汉,430070
基金项目:国家科技支撑计划(项目编号:2011BAB06B004-02)国家863科技计划(项目编号:2011AA10A201)
摘    要:十字花科根肿病是由芸薹根肿菌引起的较为严重的世界性病害之一,至今还无有效控制该病害的生物农药。本研究通过盆栽实验,筛选到一株解淀粉芽胞杆菌HB_26,对根肿病原菌具有60%以上的抑制活性。通过高效液相质谱(LC_MS)对活性物质进行分离纯化,根据分子量和紫外吸收光谱初步鉴定活性物质为肽类,命名为BA30。抑真菌实验证明BA30在150μg·mL-1的浓度下,对小麦赤霉和灰霉病菌有70%的抑菌活性,对蚕豆锈病和水稻纹枯病菌有50%的抑菌活性,对番茄早疫病菌有30%的抑菌活性。

关 键 词:十字花科根肿病  解淀粉芽胞杆菌  芸薹根肿菌  抗菌肽  分离与鉴定

Separation and Characterization of Bacillus amyloliquefaciens Active Substances Against Plasmodiophora brassicae
Affiliation:LIU Xiaoyan[1] ZHANG Wei[2] MIN Yong[1] ZHANG Yani[1] ZHANG Zhigang[1] JIANG Aibing[1] YANG Ziwen[1]
Abstract:Clubroot which is caused by Plasmodiophora brassicae,is a serious disease of brassica crops throughout the world. No any effective ways of controlling this pathogen is now available. B. amyloliquefaciens strain HB-26 with more than 60% inhibition activity against P. brassicae by Chinese cabbage pot experiment was studied. The active substance namely BA30 was separated and purified by HPLC,and it was identified to be a peptide derivative by mass spectrometry. In vitro inhibition test showed that BA30 had mutiple antifungal activity:inhibitory rate of 70% against Fusarium graminearum and Botrytis cinerea,50% against Uromyces fabae and Thanatephorus cucumeris,30% against Alternaria solani at the concentration of 150 μg·mL-1. Based on these results,we suggest that HB-26 or BA30 has potential application for the control of clubroot of brassica crops.
Keywords:clubroot  Bacillus amyloliquefaciens  Plasmodiophora brassicae  antimicrobial peptide  separation and characterization
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