Chiral HPLC analysis of milnacipran and its FMOC-derivative on cellulose-based stationary phases |
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Authors: | Patti Angela Pedotti Sonia Sanfilippo Claudia |
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Institution: | Istituto di Chimica Biomolecolare del CNR, Via del Santuario 110, I-95028 Valverde CT, Italy. angela.patti@icb.cnr.it |
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Abstract: | The HPLC enantioseparation of the last generation antidepressive drug milnacipran (+/-)-1 was investigated on different cellulose-based chiral stationary phases (CSPs). On carbamate-type columns, Chiralcel OD and OD-H (+/-)-1 could be separated with alpha value about 1.20 but the resolution was quite low because of the tailing of the peaks. Direct determination of (+/-)-1 with high selectivity and resolution was obtained on Chiralcel OJ in normal phase mode elution. Precolumn derivatization of milnacipran with Fmoc-Cl gave compound (+/-)-2 which was enantioseparated on all the investigated CSPs and allowed enhanced UV or fluorimetric detection. The Chiralpak IB, that could be considered the immobilized version of Chiralcel OD-H, was found completely ineffective in the chiral recognition of (+/-)-1 and moderately efficient in the separation of (+/-)-2. |
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Keywords: | milnacipran chiral drug enantioselective HPLC cellulose‐based chiral stationary phase Fmoc‐derivative |
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