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Eye‐mediated immune tolerance to Type II collagen in arthritis‐prone strains of mice
Authors:Shukkur M Farooq  Ashok Kumar  Hossam M Ashour
Institution:1. Department of Pharmacy Practice, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, , Detroit, MI, USA;2. Kresge Eye Institute/Dept. of Ophthalmology, Wayne State University, , Detroit, MI, USA;3. Dept. of Anatomy and Cell Biology, Wayne State University, , Detroit, MI, USA;4. Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, , Cairo, Egypt
Abstract:Type II collagen (CII) is a cartilage structural protein that plays important roles in joint function, arthritis and ageing. In studying the ability of CII to induce eye‐mediated specific immune tolerance, we have recently proven that CII is capable of inducing anterior chamber‐associated immune deviation (ACAID) in Balb/c mice. Here, we study the ability of CII to induce eye‐mediated immune tolerance in strains of mice that are prone to the induction of rheumatoid arthritis. Thus, we hypothesized that CII induces ACAID in DBA/1 mice and in C57BL/6 mice through the AC route (direct injection) or the intravenous route (adoptive transfer of in vitro‐generated CII‐specific ACAID macrophages or of CII‐specific in vitro‐generated T regulatory cells). Specific immune tolerance induction was assessed using both delayed‐type hypersensitivity (DTH) and local adoptive transfer (LAT) assays. Results indicated the ability of CII to generate CII‐specific ACAID‐mediated immune tolerance in vivo and in vitro in both DBA/1 mice and C57BL/6 mice. These findings could be beneficial in studies of immune tolerance induction using CII.
Keywords:   ACAID     peripheral tolerance  immune privilege  regulatory T cells  collagen type II  C57BL/6 mice  DBA/1 mice
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