Isolation and characterization of a serine/threonine protein kinase SOS2 gene from Brassica napus |
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Authors: | Wang Jin Wu Weisheng Zuo Kaijing Fei Jiong Sun Xiaofen Lin Juan Li Xuofeng Tang Kexuan |
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Affiliation: | School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology RD Center, Fudan University, Shanghai 200433, China. |
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Abstract: | A full-length cDNA of a new serine/threonine (Ser/Thr) protein kinase gene, designated as BnSOS2 (GenBank Acc. No.AY310413), was cloned from Brassica napus by rapid amplification of cDNA ends (RACE). The full-length cDNA of BnSOS2 was 1779 bp and contained a 1539-bp open reading frame encoding a protein of 512 amino acids. Homology analysis shows that BnSOS2 strongly resembles other Ser/Thr protein kinase genes, and that its putative protein belongs to a typical Ser/Thr kinase family. Northern blot analysis reveals that BnSOS2 is salt-inducible. Our results indicate that BnSOS2 is a new member of the plant SOS2 gene family, which may play an important role in salt tolerance of plants. |
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