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<Emphasis Type="Italic"> Zaba</Emphasis>: a novel miniature transposable element present in genomes of legume plants
Authors:Email author" target="_blank">J?MacasEmail author  P?Neumann  D?Po?árková
Institution:(1) Laboratory of Molecular Cytogenetics, Institute of Plant Molecular Biology, Braniscaronovská 31, 37005 Ccaroneské Budecaronjovice, Czech Republic
Abstract:A novel family of miniature transposable elements, named Zaba, was identified in pea (Pisum sativum) and subsequently also in other legume species using computer analysis of their DNA sequences. Zaba elements are 141–190 bp long, generate 10-bp target site duplications, and their terminal inverted repeats make up most of the sequence. Zaba elements thus resemble class 3 foldback transposons. The elements are only moderately repetitive in pea (tens to hundreds copies per haploid genome), but they are present in up to thousands of copies in the genomes of several Medicago and Vicia species. More detailed analysis of the elements from pea, including isolation of new sequences from a genomic library, revealed that a fraction of these elements are truncated, and that their last transposition probably did not occur recently. A search for Zaba sequences in EST databases showed that at least some elements are transcribed, most probably due to their association with genic regions.Electronic Supplementary Material Supplementary material is available for this article if you access the article at . A link in the frame on the left on that page takes you directly to the supplementary material.Communicated by M.-A. Grandbastien
Keywords:Miniature transposable elements  Repetitive DNA  Plant genome  Computer analysis  Pea ( Pisum sativum)
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