Interaction of puromycin with acceptor site of human placenta 80 S ribosomes |
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| Authors: | D M Graifer O S Fedorova G G Karpova |
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| Institution: | Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk. |
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| Abstract: | The complex N-AcPhe-tRNA(Phe).poly(U).80 S ribosome from human placenta was treated with puromycin taken in various concentrations. Based on the kinetic data of N-acetylphenylalanyl-puromycin formation, the association constant of puromycin with the acceptor site of the ribosome was estimated to be (3.96 +/- 0.84) x 10(4) M-1 at 37 degrees C. |
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