(1) Laboratório de Enzimologia, Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brasil, CEP 70910-900;(2) Institut für Holzchemie, Leuschnerstrabe 91, 2050 Hamburg 80, Germany
Abstract:
Xylan-degrading enzyme activities were isolated from crude extracts of solid-state cultures of Aspergillus fumigatus Fresenius (Xyl I, Xyl III, Xyl IV and Xyl V) and Humicola grisea var. thermoidea (Xyl II) by chromatographic procedures. The pattern of hydrolysis of different xylans and pulps varied from traces of xylose to xylooligomers. The products formed suggest an endo-type enzyme mode of action. Some enzymes showed debranching and transglycosidase activities.