Enzymatic hydrolysis of cyanohydrins with recombinant nitrile hydratase and amidase from <i>hodococcus erythropolis</i>期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Enzymatic hydrolysis of cyanohydrins with recombinant nitrile hydratase and amidase from hodococcus erythropolis

Authors:	Ch. Reisinger I. Osprian A. Glieder H. E. Schoemaker H. Griengl H. Schwab

Affiliation:	(1) Institute of Molecular Biotechnology, Graz University of Technology, Petersgasse 14, 8010 Graz, Austria;(2) Institute of Organic Chemistry, Graz University of Technology, Stremayrgasse 16/2, 8010 Graz, Austria;(3) Competence Centre Applied Biocatalysis, Petersgasse 14, 8010 Graz, Austria;(4) Van t Hoff Institute for Molecular Sciences, University of Amsterdam, Nieuwe Achtergracht 129, 1018 WS Amsterdam, The Netherlands

Abstract:	Nitrile hydratase and amidase from Rhodococcus erythropolis CIMB11540 were both cloned and expressed in Escherichia coli.Crude cell free extracts were used for the hydrolysis of different aromatic cyanohydrins. Nitrile hydratase expression was increased up to 5-fold by redesign of the expression cassette. The recombinant enzymes were successfully used for the conversion of several cyanohydrins to the corresponding Î±-hydroxy amides and acids while retaining enantiopurity.

Keywords:	amidase biotransformation cyanohydrin hydrolysis nitrile hydratase
本文献已被 SpringerLink 等数据库收录！