Effect of protoplast source and media on growth and regenerability of protoplast-derived calluses of Solanum tuberosum L |
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Authors: | Muhammad Akbar Anjum |
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Affiliation: | (1) Department of Horticulture, University College of Agriculture, Bahauddin Zakariya University, 60800 Multan, Pakistan |
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Abstract: | Freshly isolated mesophyll and suspensions-cell protoplasts of S. tuberosum cvs. Desiree and Maris Piper were cultured in different media i.e. modified MS, V-KM and MS-KM. Protoplast plating efficiencies were higher in MS-KM medium. Resulting protoplast-derived calluses were transferred either onto the medium of Bokelmann and Roest (1983) or that of Lam (1977) for shoot regeneration. Calluses derived from mesophyll cell protoplasts differentiated about 2 weeks earlier than calluses derived from suspension-cell protoplasts. Shoot initiation was also about 2 weeks earlier from calluses subcultured onto the former medium as compared to the latter. |
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Keywords: | Plant regeneration potato protoplast culture Solanum tuberosum |
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