Butanol production by Clostridium acetobutylicum in a continuous packed bed reactor |
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Authors: | Fabio Napoli Giuseppe Olivieri Maria Elena Russo Antonio Marzocchella Piero Salatino |
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Affiliation: | 1. Chemical Engineering Department, Università degli Studi di Napoli Federico II, P.le V. Tecchio n. 80, 80125, Naples, Italy 2. Chemical Engineering Department, School of Biotechnological Sciences, P.le V. Tecchio, n. 80, 80125, Naples, Italy
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Abstract: | In this study, we report on a butanol production process by immobilized Clostridium acetobutylicum in a continuous packed bed reactor (PBR) using Tygon® rings as a carrier. The medium was a solution of lactose (15–30 g/L) and yeast extract (3 g/L) to emulate the cheese whey, an abundant lactose-rich wastewater. The reactor was operated under controlled conditions with respect to the pH and to the dilution rate. The pH and the dilution rate ranged between 4 and 5, the dilution rate between 0.54 and 2.4 h?1 (2.5 times the maximum specific growth rate assessed for suspended cells). The optimal performance of the reactor was recorded at a dilution rate of 0.97 h?1: the butanol productivity was 4.4 g/Lh and the selectivity of solvent in butanol was 88%w. |
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