Improvement of tempe fermentations by application of mixed cultures consisting of Rhizopus sp. and bacterial strains |
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| Authors: | I Wiesel H J Rehm B Bisping |
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| Institution: | Institut für Mikrobiologie, Westf?lische Wilhelms-Universit?t Münster, Corrensstra?e 3, D-48149 Münster, Germany. Fax: + 49 251 838388, DE
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| Abstract: | Tempe fermentations using mixed cultures of Rhizopus oligosporus MS5, R. oryzae EN, Citrobacter freundii, and Brevibacterium epidermidis were investigated. Consumption of 150 g tempe, produced with a pure fungal mixed culture out of strains MS5 and EN, is sufficient
to cover the daily requirements of niacin, vitamin K, ergosterol, and tocopherol as well as half of the daily requirement
of pyridoxine, riboflavin, and biotin. Moreover, one-fourth of the recommended amount of folate is supplied. Supplementation
of the fungal inoculum with C. freundii results in tempe enriched with vitamin B12. Menachinone was produced as a typical bacterial vitamin K derivative. Metabolic activity of C.␣freundii led to an additional decrease of the α-galactosides stachyose and raffinose compared to pure fungal fermentations. No bacterial
formation of factor 2 could be observed.
Received: 31 July 1996 / Received revision: 4 October 1996 / Accepted: 14 October 1996 |
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