Solid-phase extraction and determination of ranitidine in human plasma by a high-performance liquid chromatographic method utilizing midbore chromatography |
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| Authors: | Don Farthing Kim LR Brouwer Itaf Fakhry Domenic Sica |
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| Institution: | aDivision of Clinical Pharmacology and Hypertension, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298-0160, USA;bDivision of Pharmaceutics, School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599-7360, USA |
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| Abstract: | An improved high-performance liquid chromatographic (HPLC) method utilizing solid-phase extraction (SPE) and midbore chromatography was developed for the determination of ranitidine in human plasma. A mobile phase of 20 mM K2HPO4-acetonitrile-triethylamine (87.9:12.0:0.1, v/v) pH 6.0 was used with a phenyl analytical column and ultraviolet detection (UV). The method demonstrated linearity from 25 to 1000 ng/ml in 500 μl of plasma with a detection limit of 10 ng/ml. The method was utilized in a pharmacokinetic study evaluating the effects of pancreatico-biliary secretions on ranitidine absorption. |
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| Keywords: | Ranitidine |
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