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Schistosoma japonicum: ultrastructural localization of a hemoglobinase using mercury labeled pepstatin
Authors:B J Bogitsh  K F Kirschner
Affiliation:1. Department of Biology, New Mexico State University, Las Cruces, NM 88003, USA;2. Department of Biology, Lawrence University, Appleton WI 54911, USA;3. Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Fiocruz Minas, Brazil;1. School of Science, Faculty of Health and Environmental Sciences, Auckland University of Technology, Auckland 1010, New Zealand;2. Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, Xinjiang, China;3. Key Laboratory of Medical Molecular Virology of MOE/MOH, Shanghai Medical College, Fudan University, 130 Dong An Road, Shanghai 200032, China;4. School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China;5. School of Public Health and Interdisciplinary Studies, Faculty of Health and Environmental Sciences, Auckland University of Technology, Auckland 0627, New Zealand;6. Institute of Biomedical Technology, Auckland University of Technology, Auckland 1010, New Zealand;7. Maurice Wilkins Centre for Molecular Discovery, Auckland 1010, New Zealand;8. College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518071, Guangdong Province, China;9. College of Food Engineering and Nutrition Sciences, Shaanxi Normal University, Xi’an 710119, Shaanxi Province, China;10. College of Food Science and Technology, Nanchang University, Nanchang 330031, Jiangxi Province, China;1. Instituto de Física Gleb Wataghin, Universidade Estadual de Campinas, 13083-859 Campinas (SP), Brazil;2. Universidad de la Costa, Departamento de Ciencias Naturales y Exactas, Calle 58 No. 55-66, Barranquilla, Colombia;3. Departamento de Física, Facultad de Ciencias, Universidad de Tarapacá, 1000007 Arica, Chile;4. Brazilian Nanotechnology National Laboratory, Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), 13083-970 Campinas (SP), Brazil
Abstract:Mercury labeled pepstatin was used to demonstrate the site of a pepstatin sensitive hemoglobinase in paraformaldehyde fixed adult Schistosoma japonicum. Pepstatin was covalently attached to glutathione using dicyclohexylcarbodiimide followed by addition to methyl mercury chloride. Deposition of mercury was observed in lipid-like globules and autophagic vacuoles in the gastrodermis. Control studies were negative in all instances. These results complement previous cytochemical studies on the distribution of other acid hydrolases in the gastrodermis of schistosomes. It is hypothesized that this pepstatin sensitive enzyme probably belongs to the carboxyl class of proteinases.
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