| 芯片毛细管电泳检测痕量酶活性的初步探讨 |
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| 引用本文： | 丛辉,王惠民,鞠少卿,金庆辉,贾春平,宋宏伟.芯片毛细管电泳检测痕量酶活性的初步探讨[J].生物化学与生物物理进展,2009,36(4):512-516. |
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| 作者姓名： | 丛辉 王惠民 鞠少卿 金庆辉 贾春平 宋宏伟 |
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| 作者单位： | 1. 南通大学附属医院医学检验中心,南通,226001
2. 中国科学院上海微系统与信息技术研究所,上海,200050 |
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| 基金项目： | 国家高技术研究发展计划(863)资助项目(2003AA404200). |
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| 摘 要： | 微芯片技术是基于微机电加工技术(MEMS)工艺，在芯片上完成电泳检测过程的新型技术．利用自制的微流控芯片及激光诱导荧光系统建立了痕量乳酸脱氢酶(LDH)活性的检测方法．以pH 9.4, 75 mmol/L硼酸为芯片电泳缓冲液，9.73 μmol/L乳酸钙为添加剂，整个电泳过程4 min结束，利用该法测得LDH检测限(S/N=3)为6×10-3 U/L，出峰时间和峰面积的相对标准偏差分别为5.32%和3.17%，该法操作过程简单，检测灵敏度高，在临床痕量酶检测中具有较好的应用前景．
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| 关 键 词： | 芯片毛细管电泳 氧化型辅酶Ⅰ 还原型辅酶Ⅰ 乳酸脱氧酶 |
| 收稿时间： | 9/1/2008 12:00:00 AM |
| 修稿时间： | 2008/11/6 0:00:00 |
A Method to Measure Trace Enzyme Based on Microchip Capillary Electrophoresis |
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| CONG Hui,WANG Hui-Min,JU Shao-Qing,JIN Qing-Hui,JIA Chun-Ping and SONG Hong-Wei.A Method to Measure Trace Enzyme Based on Microchip Capillary Electrophoresis[J].Progress In Biochemistry and Biophysics,2009,36(4):512-516. |
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| Authors: | CONG Hui WANG Hui-Min JU Shao-Qing JIN Qing-Hui JIA Chun-Ping and SONG Hong-Wei |
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| Institution: | Laboratory Medicine Center, Affiliated Hospital of Nantong University, Nantong 226001, China;Laboratory Medicine Center, Affiliated Hospital of Nantong University, Nantong 226001, China;Laboratory Medicine Center, Affiliated Hospital of Nantong University, Nantong 226001, China;Shanghai Institute of Microsystem and Information Technology, The Chinese Academy of Sciences, Shanghai 200050, China;Shanghai Institute of Microsystem and Information Technology, The Chinese Academy of Sciences, Shanghai 200050, China;Laboratory Medicine Center, Affiliated Hospital of Nantong University, Nantong 226001, China |
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| Abstract: | |
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| Keywords: | microchip capillary electrophoresis nicotinamide adenine dinucleotide(NAD+) reduced nicotinamide adenine dinucleotide(NADH) lactate dehydrogenase |
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