Precise centromere mapping using a combination of repeat junction markers and chromatin immunoprecipitation-polymerase chain reaction |
| |
| Authors: | Luce Amy C Sharma Anupma Mollere Oliver S B Wolfgruber Thomas K Nagaki Kiyotaka Jiang Jiming Presting Gernot G Dawe R Kelly |
| |
| Affiliation: | Department of Plant Biology, University of Georgia, Georgia 30602, USA. |
| |
| Abstract: | Centromeres are difficult to map even in species where genetic resolution is excellent. Here we show that junctions between repeats provide reliable single-copy markers for recombinant inbred mapping within centromeres and pericentromeric heterochromatin. Repeat junction mapping was combined with anti-CENH3-mediated ChIP to provide a definitive map position for maize centromere 8. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
