Structural-functional changes in root cells under the action of carbonylcyanide 3-chlorphenylhydrazone

Structural and functional changes in wheat root cells under the action of protonophores-carbonyl cyanide m-chlorophenylhydrazone (CCCP) were studied. After addition of 0.5 mM CCCP we observed K+ ions uptake from the incubation medium, stimulation of O2 uptake which correlated with the occurrence of condensed mitochondria in the cells (1, 4, 6 h of incubation), and an insignificant increase of heat in roots. Taking into account the protonophoric properties of CCCP, we assume what the observed changes may be associated with the activation of a reverse energy dependent transport of H+ ions from the cytoplasm, due partially to H(+)-ATPase function intensification. The addition of diethylstibestrol (DES), a specific inhibitor of plasma membrane H(+)-ATPase, completely eliminated the stimulating effect of CCCP, that confirms the earlier assumption.