Pervasive and cooperative deadenylation of 3'UTRs by embryonic microRNA families |
| |
Authors: | Wu Edlyn Thivierge Caroline Flamand Mathieu Mathonnet Geraldine Vashisht Ajay A Wohlschlegel James Fabian Marc R Sonenberg Nahum Duchaine Thomas F |
| |
Institution: | Rosalind and Morris Goodman Cancer Research Centre, McGill University, Montreal H3A 1A3 QC, Canada. |
| |
Abstract: | To understand how miRNA-mediated silencing impacts on embryonic mRNAs, we conducted a functional survey of abundant maternal and zygotic miRNA families in the C. elegans embryo. We show that the miR-35-42 and the miR-51-56 miRNA families define maternal and zygotic miRNA-induced silencing complexes (miRISCs), respectively, that share a large number of components. Using a cell-free C. elegans embryonic extract, we demonstrate that the miRISC directs the rapid deadenylation of reporter mRNAs with natural 3'UTRs. The deadenylated targets are translationally suppressed and remarkably stable. Sampling of the predicted miR-35-42 targets reveals that roughly half are deadenylated in a miRNA-dependent manner, but with each target displaying a distinct efficiency and pattern of deadenylation. Finally, we demonstrate that functional cooperation between distinct miRISCs within 3'UTRs is required to potentiate deadenylation. With this report, we reveal the extensive and direct impact of miRNA-mediated deadenylation on embryonic mRNAs. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|