Fractionation and isolation of heparan sulfates using poly-L-lysine-Sepharose |
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Authors: | S Suzuki A Koide |
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Institution: | Tsukuba Research Laboratory, Eisai Company, Ltd., 1,3 Tokodai 5 chome, Toyosato-machi, Tsukuba-gun, Ibaragi 300-26, Japan |
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Abstract: | A simple procedure for the isolation of heparan sulfates from pig lung using a poly-L-lysine-Sepharose column is described. Glycosaminoglycans are absorbed on poly-L-lysine-Sepharose at pH 7.5 and eluted with an NaCl linear gradient in the following order: hyaluronic acid (0.32 M NaCl), chondroitin (0.36 M NaCl), keratan sulfate (0.80 M NaCl), chondroitin 4-sulfate (0.86 M NaCl), chondroitin 6-sulfate (0.95 M NaCl), dermatan sulfate (0.91 M NaCl), heparan sulfate (1.2 M NaCl), and heparin (1.35 M NaCl). Based on these observations, isolation of heparan sulfate from pig lung crude heparan sulfate fractions which contain chondroitin sulfates and dermatan sulfate was attempted, using this chromatographic technique. |
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Keywords: | glycosaminoglycans heparan sulfate Pig lung low sulfated heparan sulfate Pig lung high sulfated heparan sulfate |
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