Suppressive effect of l-phenylalanine on lignin peroxidase in the white-rot fungus Phanerochaete chrysosporium |
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Authors: | Yasumi Akamatsu Mikio Shimada |
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Affiliation: | Hans-Knöll-lnstitut für Naturstoff-Forschung e.V., Beutenbergstraβe 11, D-07745 Jena, Germany; Klinikum der Friedrich-Schiller-Universität, Institut für Ultrastrukturforschung, Ziegelmühlenweg 1, D-07743 Jena, Germany |
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Abstract: | Abstract Guanosine-5'-diphosphate-3'-diphosphate (ppGpp), an effector for many metabolic pathways, is synthesized by the relA gene product after amino acid limitation. Studies of stringent controlled Escherichia coli CP78 (relA+) and relaxed controlled E. coli CP79 (relA−) were carried out to test whether these strains differ in the appearance of their cytoplasmic membranes after induction of stringent and relaxed response. Cytoplasmic membrane structures of the cells were investigated by freeze-fracture electron microscopy after cooling the cells. The obtained micrographs showed a net-like distribution of the particles in the cytoplasmic membranes of relaxed controlled cells whereas such a pattern was not detectable in the stringent controlled counterparts. |
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Keywords: | Freeze fracturing Stringent and relaxed response Escherichia coli |
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