卟啉化合物四-[3-甲氧基-4-(N-咔唑)]正丁氧苯基和四-[3-甲氧基-4-(N-咔唑)]正己氧苯基与小牛胸腺DNA相互作用的研究 |
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引用本文: | 苏彦文,吴雷,吕明,王雪丽,苏彦斌,李正强. 卟啉化合物四-[3-甲氧基-4-(N-咔唑)]正丁氧苯基和四-[3-甲氧基-4-(N-咔唑)]正己氧苯基与小牛胸腺DNA相互作用的研究[J]. 生物物理学报, 2009, 25(6): 405-414 |
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作者姓名: | 苏彦文 吴雷 吕明 王雪丽 苏彦斌 李正强 |
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作者单位: | 1. 吉林化工学院 2. 吉林大学分子酶学工程教育部重点实验室 |
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摘 要: | 〔四-[3-甲氧基-4-(N-咔唑)正丁氧苯基],4C4-TPP〕和〔四-[3-甲氧基-4-(N-咔唑)正己氧苯基],4C6-TPP〕是两个结构相似但侧链不同的卟啉化合物,4C6-TPP的侧链长于4C4-TPP。应用紫外吸收光谱、荧光光谱和园二色谱,研究了4C4-TPP和4C6-TPP与小牛胸腺DNA(Calf thymus, ctDNA)之间的相互作用。结果表明:4C4-TPP和4C6-TPP均以侧链插入DNA与之作用,计算二者与DNA之间的结合常数,4C6-TPP与DNA的结合常数远大于4C4-TPP与DNA的结合常数。基于4C4-TPP与4C6-TPP二者之间结构差异仅在于侧链基团,证明了侧链基团对于卟啉与DNA作用的影响不是主要决定于其空间尺寸大小,取代基化学结构是影响卟啉与DNA的相互作用的重要因素。
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关 键 词: | 卟啉 小牛胸腺DNA 园二色谱 结合常数 |
收稿时间: | 2009-09-08 |
修稿时间: | 2009-11-29 |
Interaction of [3-Methoxy-4- (N-Carbazyl)]N-BuxyloxyPhenyl]Porphyrin and [3-Methoxy-4- (N-Carbazyl)N-Hexyloxyphenyl]Porphyrin with ctDNA |
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Affiliation: | 1. Jilin Institute of Chemical Technology, Jilin 132022, China; 2. Key Laboratory for Molecular Enzymology and Engineering of Educational Ministry, Jilin University, Changchun 130021, China |
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Abstract: | 4C4-TPP(meso-tetrakis[3-methoxy-4-(N-carbazyl)n-buxyloxyphenyl]porphyrin) and 4C6-TPP (meso-tetrakis [3-methoxy-4-(N-carbazyl)n-hexyloxyphenyl]porphyrin) are similar in structure, but the substitutent size of later is longer than that of former. The interaction of 4C4-TPP and 4C6-TPP with calf thymus-DNA (ctDNA) was explored by UV-vis, fluorescence and circular dichroism spectra. The results demonstrated that the outside of 4C4-TPP and 4C6-TPP binds ctDNA but peripheral substitutents intercalated. The binding constant of 4C6-TPP with ctDNA is greater than that of 4C4-TPP. The difference of binding between 4C4-TPP and 4C6-TPP suggests that the chemical structure of peripheral substitutent plays a more important role than merely their size in determining the binding preferences of porphyrins. |
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Keywords: | Porphyrin Calf thymus-DNA Circular Dichroism Binding constant |
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