Ca2(+)-binding site of carp parvalbumin recognized by monoclonal antibody. |
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Authors: | R Tinner M Oertle C W Heizmann H R Bosshard |
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Affiliation: | Biochemisches Institut, Universit?t Zürich, Switzerland. |
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Abstract: | Monoclonal antibody 235 which was used for immunohistochemical staining of parvalbumin in tissue sections partially protects Lys-54 of carp muscle parvalbumin from reaction with acetic anhydride in the parvalbumin-antibody complex. Lys-54 is located in the CD-loop of parvalbumin and is flanked by the Ca2(+)-ligands Asp-53 and Ser-55 of the Ca2(+)-site I. Another monoclonal antibody against carp parvalbumin, mca 239, partially protects lysine residues 27, 32, 87 and 107, indicating that this antibody is directed against a discontinuous epitope distant from the two Ca2(+)-binding sites of parvalbumin. |
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