Electron microscopy of phages for Agrobacterium tumefaciens |
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Authors: | R. J. Boyd A. C. Hildebrandt O. N. Allen |
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Affiliation: | (1) Department of Bacteriology, University of Wisconsin, U.S.A.;(2) Department of Plant Pathology, University of Wisconsin, U.S.A. |
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Abstract: | Summary Bacteriophages for three strains of A. tumefaciens were concentrated by ultracentrifugation, stained with 1% phosphotungstic acid (PTA), or 0.5% uranyl acetate, and examined with the electron microscope. Phage PT11 was a bacillary-shaped particle with a whip-like tail containing a knob at its distal end. Phage PIIBNV6 appeared to have an icosahedral head. The wide non-contractile tail terminated in a plate with pegs. Phage PIIBNV6-C was an icosahedral particle with a short, spike-like tail. Host cells of A. tumefaciens were encapsulated rods bearing polar or lateral flagella.Published with approval of the Director, Wisconsin Agricultural Experiment Station, Madison, Wisconsin, U.S.A. 53706. |
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