Design and application of a new cryptic-plasmid-based shuttle vector for Magnetospirillum magneticum |
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| Authors: | Okamura Yoshiko Takeyama Haruko Sekine Takumi Sakaguchi Toshifumi Wahyudi Aris Tri Sato Rika Kamiya Shinji Matsunaga Tadashi |
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| Affiliation: | Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Koganei, Tokyo. |
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| Abstract: | A 3.7-kb cryptic plasmid designated pMGT was found in Magnetospirillum magneticum MGT-1. It was characterized and used for the development of an improved expression system in strain AMB-1 through the construction of a shuttle vector, pUMG. An electroporation method for magnetic bacteria that uses the cryptic plasmid was also developed. |
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