红厚壳茎段丛生芽诱导与植株再生

红厚壳(Calophyllum inophyllum)为藤黄科红厚壳属多年生木本植物,有很高的药用价值。该研究以红厚壳带节茎段为外植体,探讨生长调节剂对腋芽萌发及丛生芽诱导、伸长和试管苗生根的影响。研究结果表明,外植体腋芽萌发和丛生芽诱导效果最好的培养基是MS+NAA1.0+TDZ0.5,在此条件下培养21天后,转入添加0.5 g·L–1活性炭且无生长调节剂的MS培养基,可有效促进不定芽的伸长。将带不定芽的外植体先在附加1.0 mg·L–1NAA的1/2MS培养基上进行生根诱导4周,之后转入附加1.0 g·L–1活性炭的无激素培养基进行根的伸长培养,这样的两步生根法能有效促进红厚壳生根。

Axillary Bud Propagation and Regeneration from Stem Segment Explants in Calophyllum inophyllum

Calophyllum inophyllum is an important medicinal tree belonging to the Guttiferae family. We used stem segments with a node as explants for bud propagation and plantlet regeneration and examined the effects of plant growth regulators on axillary bud germination, propagation, bud elongation and rooting from adventitious buds. The suitable medium for axillary bud germination and propagation was MS supplemented with NAA1.0 and TDZ0.5. Culture of explants on this medium for 21 days and then transfer to hormone-free medium supplied with 0.5 g·L-1 active carbon promoted the elongation of adventitious buds. We developed a two-step method for rooting. The explants with adventitious buds were first cultured on 1/2 MS medium with 1 mg·L-1NAA for 4 weeks, then on hormone-free medium supplied with 1.0 g·L^-1 active carbon. This two-step rooting method accelerated rooting efficiently.