Staining Whole Mounts of an Insect (Tribolium confusum) Reproductive System for Succinic Dehydrogenase and nonspecific esterase |
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Authors: | Anilbaran Raychaudiuri |
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Institution: |
a Aging-Research Laboratory, Veterans Administration Hospital, Baltimore, Md |
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Abstract: | The female reproductive system of Tribolium confusum was dissected in a salt solution containing NaC1, 9 gm; KC1, 0.42 gm; and CaC12, 0.25 gm per liter and fixed in 1% CaC12-10% formol, both at 0-4 C, or air dried. Staining was by standard histochemical procedures. Selective intensity of enzyme activity was as follows: dehydrogenase was predominant in the ovarioles; esterase, in the lateral oviducts. The use of iodoacetic acid and L600 as inhibitors suppressed the dehydrogenase and nonspecific esterase activity, respectively. |
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