Inductive effect produced by a mixture of carbon source in the production of gibberellic acid by Gibberella fujikuroi |
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Authors: | Erika Y Rios-Iribe Luis B Flores-Cotera Mario M González Chávira Guillermo González-Alatorre and Eleazar M Escamilla-Silva |
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Institution: | (1) Facultad de Qu?mica, Universidad Aut?noma de Quer?taro, Cerro de las Campanas S/N, Col. Las Campanas Apartado Postal 184, C.P. 76010 Quer?taro, Quer?taro, Mexico;(2) Departamento de Biotecnolog?a y Bioingenier?a, Centro de Investigaci?n y Estudios Avanzados del Instituto Polit?cnico Nacional, Ave. Polit?cnico 2508, C.P. 07360 Mexico DF, Mexico;(3) Laboratorio de Biotecnolog?a, Instituto Nacional de Investigaciones Forestales, Agr?colas y Pecuarias. Km 6.5 Carretera Celaya-San Miguel de Allende S/N. Col. Roque, C.P. 38110 Celaya, Guanajuato, Mexico;(4) Departamento de Ingenier?a Qu?mica, Instituto Tecnol?gico de Celaya, Av. Tecnol?gico y Antonio Garc?a Cubas S/N, C.P. 38010 Celaya, Guanajuato, Mexico; |
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Abstract: | Gibberellic acid has been known since 1954 but its effect on rice still remains very important in the agricultural world.
Gibberellic acid (GA3) is the main secondary metabolite produced by the Gibberella fujikuroi fungus. This hormone is of great importance in agriculture and the brewing industry, due to its fast and strong effects at
low concentrations (μg) on the processes of growth stimulation, flowering, stem elongation, and germination of seeds, among
others. Plant promoters of growth production such as the gibberellins, especially the GA3 are a priority in obtaining better harvests in the agricultural area and by extension, improving the food industry. Three
routes to obtaining GA3 have been reported: extraction from plants, chemical synthesis and microbial fermentation. The latter being the most common
method used to produce GA3. In this investigation, glucose-corn oil mixture was used as a carbon source on the basis of 40 g of carbon in a 7 L stirred
tank bioreactor. A pH of 3.5, 29°C, 600 min−1 agitation and 1 vvm aeration were maintained and controlled with a biocontroller connected to the bioreactor, throughout
the entire culture time. The carbon source mixture affected the fermentation time as well as the production of the GAs. The
production of 380 mg GA3L−1 after 288 h of fermentation was obtained when the glucose-corn oil mixture was employed contrasting the 136 mg GA3L−1 at 264 h of culture when only glucose was used. |
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