Further resolution of the quail karyotype and characterization of microchromosomes by counterstain-enhanced fluorescence |
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| Authors: | B Mayr M Lambrou W Schleger |
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| Affiliation: | Institute for Animal Breeding and Genetics, Veterinary University, Vienna, Austria. |
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| Abstract: | Sequential staining with a counterstain-contrasted fluorescent R-banding technique (chromomycin A3/distamycin A-DAPI) followed by DAPI-actinomycin D-induced quinacrine-fluorescence-Hoechst 33258 (QFH)-type banding allowed the identification of quail chromosomes up to chromosome 19. The chromomycin A3-positive staining behavior of the W chromosome and of the heterochromatic areas of most microchromosomes indicated their GC-rich nature. |
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