L-alanine transport in isolated cells of interscapular brown adipose tissue in rat |
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Authors: | A Rodríguez-Martín X Remesar |
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Institution: | (1) Unitat de Bioquímica i Biologia Molecular B. Departament de Boquímica i Fisiologia, Universitat de Barcelona, 08071 Barcelona, Spain |
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Abstract: | The pattern of L-alanine uptake in isolated cells of interscapular brown adipose tissue has been determined. The uptake can be divided into the diffusion component (Kd=0.55 min–1) and a saturable Na+-dependent transport (K
M
=0.87 mM andV
max=155 nmol/min/106 cells). The saturable component can be subdivided into MeAIB-sensitive (K
M
=1.63 mM andV
max=162 nmol/min/106 cells) and MeAIB-insensitive (K
M
=3.2 mM andV
max=39.5 nmol/min/106 cells). This kinetic pattern could indicate the presence of transport system (s) that resemble the commonly described transport systems for alanine uptake in several tissues.Abbreviations MeAIB
Methyl-aminoisobutyric acid
- AIB
Aminoisobutyric acid |
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Keywords: | alanine brown adipose cells transport |
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