Effect of cultural conditions on the lipid profile of Thiobacillus ferroxidas |
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Authors: | Madhumita Ghosh Ajit K. Mishra |
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Affiliation: | (1) Department of Microbiology, Bose Institute, 700009 Calcutta, India |
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Abstract: | The intensitive investigations on the lipid profile of Thiobacillus ferrooxidans at various culture ages suggest some correlations of the lipid constitutents with the membrane-bound iron oxidation system. Phosphatidic acid, phosphatidyl serine and phosphatidyl ethanolamine were the major polar components; hydrocarbon, triglyceride and diglyceride were the main neutral components. Major fatty acids were C16:0, C16:1, C16:3, C18:1, C18:3, C22:1 while C20:1, C20:2, C12:0, C14:2, C18:0, C18:2, C20:0, C22:0 were found in trace amounts which also depended upon the phase of the growth. One lipoamino acid was identified as ornithine lipid in the polar fraction. Each and every component varied to some extent at different growth phasesindicating relationship of these lipids to the iron oxidation system of the strain. |
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Keywords: | Thiobacillus ferrooxidans Lipids Ornithine lipid Ubiquinone Behenic acid Iron oxidation system Fatty acids |
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