Monoclonal antibodies to the murine zona pellucida protein with sperm receptor activity: effects on fertilization and early development |
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| Authors: | I J East B J Gulyas J Dean |
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| Affiliation: | 1. Ohio State University Comprehensive Cancer Center, Columbus, OH, United States;2. GNOME Diagnostic Laboratory, Powell, OH 43065, United States;1. Laboratory of Reproduction Medicine, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian, China;2. Department of Surgery, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian, China;3. Department of Obstetric and Gynecology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian, China;4. Department of Clinical Laboratory, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian, China;1. Metabolism & Genetics Group, Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Portugal;2. Department of Biochemistry and Human Biology, Faculty of Pharmacy, Universidade de Lisboa, Portugal;3. Department of Human Genetics, Molecular Cytogenetic Unit, National Institute of Health Doctor Ricardo Jorge, I.P., Lisbon, Portugal;4. Department of Medicine, Hospital Santa Maria, Lisbon, Portugal;5. Department of Pediatrics, Hospital Santa Maria, Lisbon, Portugal |
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| Abstract: | During development and maturation, mammalian oocytes are surrounded by the zona pellucida which in the mouse is comprised of three sulfated glycoproteins, ZP-1, ZP-2, and ZP-3. Previously, monoclonal antibodies to ZP-2 have been isolated. The isolation and characterization of monoclonal antibodies specific for ZP-3, the zona protein with sperm receptor activity are now reported. Following passive immunization, these monoclonal antibodies localize to the intraovarian zonae pellucidae and their presence precludes both in vivo and in vitro fertilization of subsequently ovulated eggs. Monoclonal antibodies specific for either ZP-2 or ZP-3 also completely block in vitro fertilization at relatively low concentration ranging from 0.4 to 75 micrograms/ml. The contraceptive effect requires the presence of the zona and appears to inhibit the penetration of the zona pellucida by sperm rather than by blocking the sperm binding site. Neither antibody interferes with in vitro development from the two-cell to the blastocyst stage or with subsequent hatching from the enveloping zona pellucida. |
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