Enhanced concentration and isolation of Cyclospora cayetanensis oocysts from human fecal samples |
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| Authors: | Riner Diana K Mullin Andrew S Lucas Sasha Y Cross John H Lindquist H D Alan |
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| Institution: | Pegasus Technical Services Inc., 46 E. Hollister St. Cincinnati, OH 45219, United States. |
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| Abstract: | Cyclospora cayetanensis is the causative agent of cyclosporiasis, an emerging infectious disease. We present a new method for the purification of C. cayetanensis oocysts from feces using a modified detachment solution and Renocal-sucrose gradient sedimentation. This method yields oocysts free from adherent fecal debris and amenable to processing using flow cytometry. |
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