Mixotrophic and heterotrophic growth of Beggiatoa alba in continuous culture |
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Authors: | Hans Güde William R Strohl John M Larkin |
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Institution: | (1) Abteilung Mikrobiologie, Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300 Braunschweig-Stöckheim, Germany;(2) Department of Microbiology, Louisiana State University, 70803 Baton Rouge, Louisiana, USA;(3) Present address: Institut für Seenforschung, Untere Seestr. 81, D-7994 Langenargen, Germany;(4) Present address: Department of Microbiology, Ohio State University, 484 West 12th Ave., 43210 Columbus, Ohio, USA |
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Abstract: | Beggiatoa alba strain B18LD was grown in continuous culture under heterotrophic conditions on acetate or acetate and asparagine and under mixotrophic conditions on acetate plus either 1 mM sodium sulfide or 1 mM sodium thiosulfate. Considerable differences were observed between the yields and the cell compositions of heterotrophic and mixotrophic cultures at all dilution rates tested. The dry weight yield per gram acetate utilized was approximately three times higher in the acetate-sulfide mixotrophic culture than in the acetate heterotrophic culture, whereas the poly--hydroxybutyric acid and carbohydrate contents were much higher in the heterotrophic cultures. The high yields (0.52–0.75, corrected for the weight of the sulfur) obtained with the mixotrophic cultures imply that the acetate was utilized mainly for biosynthesis. Thus, the oxidation of sulfide supplied energy. The addition of catalase to the chemostat cultures increased yields slightly, but it was insufficient to explain the differences between the heterotrophic and the mixotrophic cultures. |
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Keywords: | Beggiatoa alba Continuous culture Chemostat Mixotrophic growth Trichomes Growth yields Sulfide oxidation |
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