Studies on the determination of urinary estrogens; the elimination of impurities in urinary estrogens by physico-chemical means |
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| Authors: | CAUGHEY M R GRAUER R C STRICKLER H S |
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| Affiliation: | 1. Univ Hassan 1, Laboratoire des Sciences des Matériaux, des Milieux et de la Modélisation (LS3M), BP.145, 25000 Khouribga, Morocco;2. Equipe de Recherche Analyse Contrôle et Environnement (ERACE), EST Safi, BP: 89, Route Dar Si Aissa, Safi, Morocco;3. Laboratoire de Catalyse et Corrosion des Matériaux, Faculté des Sciences, Université Chouaib Doukkali, BP: 20, 24000 El Jadida, Morocco;4. LMPEQ, ENSA Safi, Université Cadi Ayyad, Sidi Bouzid, B.P. 63, Safi 46000, Morocco;5. GEMH, Ecole Nationale Supérieure de Céramique Industrielle, Centre Européen de la Céramique, 12 rue Atlantis, 87068 Limoges cedex, France;1. Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China;2. Molecular Oncology and Epigenetics Laboratory, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China;3. Department of Chemistry, University of California, Riverside, CA 92521, USA;1. Key Laboratory of Road and Traffic Engineering of Ministry of Education, Tongji University, 304 Tongda Building, 4800 Cao’an Road, Shanghai 201804, China;2. Key Laboratory of Road and Traffic Engineering of Ministry of Education, Tongji University, 608 Tongda Building, 4800 Cao’an Road, Shanghai 201804, China;1. Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China;2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100193, China;3. Shunyi Center for Animal Disease Control and Prevention, Beijing 101300, China |
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| Abstract: | Urinary extracts were purified by Engel's method and countercurrent distribution, chromatography, and redistribution. Methods of estimating impurity levels and quenching were developed. Optimum conditions of sensitizing extracts were determined for use of a correction equation based on decrease of fluorescence of estrogen and constancy of that of impurities. |
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