DNA Methylation Signature Analysis: How Easy Is It to Perform? |
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| Authors: | Christina Piperi Elena Farmaki Fotis Vlastos Athanasios G Papavassiliou Nadine Martinet |
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| Institution: | 1. Department of Biological Chemistry, Medical School, University of Athens, Athens, Greece;2. Department of Respiratory Medicine, ‘Sotiria’ General Hospital, Athens, Greece;3. Centre de Ressources Biologiques, INSERM, Unité 724, Nancy, France |
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| Abstract: | Epigenetic changes, or heritable alterations in gene function that do not affect DNA sequence, are rapidly gaining acceptance as co-conspirators in carcinogenesis. Although DNA methylation signature analysis by methylation-specific polymerase chain reaction has been a breakthrough method in speed and sensitivity for gene methylation studies, several factors still limit its application as a routine diagnostic and prognostic test. |
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| Keywords: | cancer diagnosis carcinogenesis DNA methylation epigenetic change methylation-specific PCR |
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